They have an inherent variability in the amount and location of dye that covalently binds to the protein this produces diffuse, broader bands than those of the recombinant prestained standards and the resulting molecular weights vary from lot to lot. Traditional prestained proteins, on the other hand, are blended from naturally occurring proteins. The staining process has been optimized to guarantee the same electrophoretic molecular weight with each lot. Recombinant prestained protein standards are carefully engineered for precise and accurate molecular weights. Product Description Precision Plus Protein Kaleidoscope standards contain ten protein bands of 10, 15, 20, 25, 37, 50, 75, 100, 150, and 250 kD. >0.99 are typical for Precision Plus Protein Kaleidoscope standards. Standards determined on a Criterion 4–20% gel. ![]() Plot for Precision Plus Protein Kaleidoscope Stored as directed for longer than 12 months, the shelf life may be extended by adding dithiothreitol (DTT) to 50 mM Large (18.3 W x 19.3 L cm) gel electrophoresisģ0% (w/v) glycerol, 2% SDS, 62.5 mM Tris,ġ2 months at –20☌ if the product has been (13.3 W x 8.7 L cm) gels, monitoring transfer ![]() Do not heat the product above room temperature.īlotting mini (8.3 W x 6.4 L cm) and midi Have precipitated have returned to solution. This will ensure that any solids that may Allow the tube to reach room temperature and thoroughly mix before use. Instructions for Use Precision Plus Protein Kaleidoscope standardsĪre provided in loading buffer, ready to load, with no dilution required.
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